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The key signaling pathways investigated include the nuclear factor erthriod 2 related factor 2 (Nrf2) activation cascade which leads to the upregulation of phase 2 detoxification genes heme oxygenase (HO1), superoxide dismutase and catalase (CAT), inhibition of the inflammatory signaling pathway of nuclear kappa B (NF-B), modulation of PI3K/Akt/mTOR pathways in cancer cells and activation of the adenosine monophosphate activated protein kinase (AMPK) for metabolic regulation

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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator